question archive The sandwich ELISA differs slightly from the ELISA we used in the disease transmission lab

The sandwich ELISA differs slightly from the ELISA we used in the disease transmission lab

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The sandwich ELISA differs slightly from the ELISA we used in the disease transmission lab. Instead of coating the plastic wells with an antigen, the wells are coated with a primary antibody (capture antibody) to bind antigen present in the sample. The secondary antibody attached to a color- changing substrate is then added to bind to the captured antigen. Which of the images below depicts the primary antibody, secondary antibody, and antigen in a sandwich ELISA? Substrate Substrate Substrate Substrate Secondary Antibody Conjugate Inhibitor Antigen Primary Antibody Conjugate Ag Capture Antibody A B D OC OD O A O B

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