question archive The sandwich ELISA differs slightly from the ELISA we used in the disease transmission lab
Subject:BiologyPrice: Bought3
The sandwich ELISA differs slightly from the ELISA we used in the disease transmission lab. Instead of coating the plastic wells with an antigen, the wells are coated with a primary antibody (capture antibody) to bind antigen present in the sample. The secondary antibody attached to a color- changing substrate is then added to bind to the captured antigen. Which of the images below depicts the primary antibody, secondary antibody, and antigen in a sandwich ELISA? Substrate Substrate Substrate Substrate Secondary Antibody Conjugate Inhibitor Antigen Primary Antibody Conjugate Ag Capture Antibody A B D OC OD O A O B