question archive Assume the DpnI digestion has failed after the PCR in the synthesis of mutated plasmid by thermal cycling process
Assume the DpnI digestion has failed after the PCR in the synthesis of mutated plasmid by thermal cycling process
Subject:BiologyPrice: Bought3
Assume the DpnI digestion has failed after the PCR in the synthesis of mutated plasmid by thermal cycling process. Under such circumstance,
a) What kind of transformants would be found on the plate? Provide reason(s) for your answer.
b) How would it affect the number of colonies that contain the mutant construct? Provide reason(s) for your answer.
