question archive Assume that the PCR protocol was repeated using person #3's DNA
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Assume that the PCR protocol was repeated using person #3's DNA. The forward primer, reverse primer, and all other PCR reagents were added to the mixture, but a second forward primer was also added to the tube. This second forward primer is designed to bind the template 400bp before the original forward primer. Explain what would happen if this second primer was added, and explain how the gel would appear.