question archive Describe the chemical mechanism for the formation of a phosphodiester bond

Describe the chemical mechanism for the formation of a phosphodiester bond

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Describe the chemical mechanism for the formation of a phosphodiester bond. What are the nucleophile, the electrophile, and the leaving group in this reaction? Why is this reaction energetically favorable, and why is the reaction effectively irreversible? Would you argue that one enzyme can catalyze both phosphodiester breakdown and synthesis, or does each process require a separate enzyme? Can you explain how this reaction is driven forward by both coupling and Q and is thus essentially irreversible?

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