question archive One of the original mutations isolated by Jacob and Monod was O0 (also called O-), often caused by single bp changes

One of the original mutations isolated by Jacob and Monod was O0 (also called O-), often caused by single bp changes

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One of the original mutations isolated by Jacob and Monod was O0 (also called O-), often caused by single bp changes. The O0 mutant fails to express B-gal and permease under any condition. Although we now connect the symbol O, operator designation to the binding site of the lac repressor, originally the O locus encompassed the whole lac promoter region. It was shown later that the O0 mutant can bind both CAP and the lacI product. What positive regulator was affected by the O0 mutation and what could a single bp change affect in this mutant?

 

 

a) Binding by RNA polymerase to the lac promoter region. The bp could disrupt the -35 or -10 consensus regions.

 

b) The ribosome. The Shine-Delgarno site (the RNA motif bound by the ribosome).

 

c) The rho terminator. The termination site for the operon.

 

d) The lacI gene promoter. The Shine-Delgarno sequence (the RNA motif recognized by the ribosome)

 

e) The lacI coding sequence. The allolactose binding site.

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