question archive How do you confirm that the chosen alcohol dehydrogenase is most efficient at the conversion of acetaldehyde to ethanol by using enzyme kinetics? No real data is given, describe what Km and Vmax you might expect to see (simulate data if you want)
How do you confirm that the chosen alcohol dehydrogenase is most efficient at the conversion of acetaldehyde to ethanol by using enzyme kinetics? No real data is given, describe what Km and Vmax you might expect to see (simulate data if you want)
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How do you confirm that the chosen alcohol dehydrogenase is most efficient at the conversion of acetaldehyde to ethanol by using enzyme kinetics?
No real data is given, describe what Km and Vmax you might expect to see (simulate data if you want)
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Answer:
Alcohol Dehydrogenase Isoenzymes
Alcohol dehydrogenase oxidizes ethanol and reduces nicotinamide adenine dinucleotide (NAD+) to NADH (Höög & Ostberg, 2011; Kalant, 2004). ADH is a zinc metalloprotein with five classes of isoenzymes that arise from the association of eight different subunits into dimers. These five classes of ADH are the products of five gene loci (ADH1-5). Class 1 isoenzymes generally require a low concentration of ethanol to achieve 'half-maximal activity′ (low Km), whereas class 2 isoenzymes have a relatively high Km. Class 3 ADH has a low affinity for ethanol and does not participate in the oxidation of ethanol in the liver. Class 4 ADH is found in the human stomach and class 5 has been reported in liver and stomach. Whereas the majority of ethanol metabolism occurs in the liver, gastric ADH is responsible for a small portion of ethanol oxidation.
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