ABO Blood Typing Lab All blood and blood sera in this kit are simulated and contain no blood or blood products. These materials cannot be used to type actual human blood. If human anti-sera were available, however, similar instructions could be used to type actual human blood. Each student will type one sample of blood, labeled Jane Smith, John Smith, David Smith, or Wiley Smith. You will need one Hema-tag label, one microscope slide, one green and one yellow toothpick. Take your turn to follow the steps outlined below. After using the slide, wash it with tap water, dry it, and return it for others to use. 1. Pick a bottle of blood from one of the four individuals; perform the test at room temperature. 2. Lay a microscope slide over the anti-A and anti-B circles on a Hema-tag 3. Place 1 drop of anti-A and 1 drop of anti-B in their respective circles on the microscope slide. 4. Add 1 drop of the blood being tested to each drop of antiserum. 5. Mix the simulated blood and antisera with a designated toothpick within the respective circles, scraping the toothpick firmly against the microscope slide i the process. 6. Continue stirring for two minutes or until agglutination is noted 7. Read for gross agglutination and record test results. Note that in this simulation precipitates are recorded as agglutinations, or positive reactions. 8. Keep microscope slide with results for extension (optional). 9. Rinse microscope slide, and dry. Repeat this same procedure for the remaining three blood samples. Lab Data: Individual: Reaction in Anti-A (+/-): Reaction in Anti-B (+/-): Blood Type: Jane Smith John Smith gahgen bluco an David Smith Wiley Smith O V Post Lab Questions: 1. These four orphans are trying to find their blood brothers or sisters from whom they were separated while very young. John Smith knows that his father was blood type B. Could John have the same father as Wiley Smith? Explain. for bould words stanab In

2. Could these four orphans be related? Explain. boold bris book i'llA indal boold 3. If Jane Smith is heterozygous for her blood type, and she marries someone who is homozygous for the blood type with two B alleles, what possible blood types could their children have? :ated dal naime enel 4. Given that Jane is heterozygous, what blood types could her offspring have if she marries someone with type 0 blood? 5. What blood types could each individual donate their blood to? a. Jane Smith b. John Smith c. David Smith d. Wiley Smith

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