Please explain what this means RESTRICTION ENDONUCLEASE DIGESTION OF PLASMID DNA? How is agarose electrophoresis involved? We used pbr 322 plasmid and Nine (9) restriction endonucleases were used to cut the pBR322 plasmid. These include BamHI, EcoRI, ScaI, HindIII, PstI, PvuII, ClaI, SapI, SalI. 

 

The process in which DNA is cut at specific sites is called the restriction endonuclease digestion.

Agarose gel electrophoresis takes place in resolving DNA fragments based on their molecular weight.

Step-by-step explanation

Restriction endonuclease digestion is done by incubating the target DNA molecule with restriction enzymes (restriction enzymes are enzymes that recognize and bind specific DNA sequences and cleave at specific nucleotides) Restriction endonuclease digestion is commonly used for molecular cloning, as the procedure allows fragments of DNA to be pieced together like building blocks via ligation.

Agarose gel electrophoresis shows that smaller DNA fragments migrate faster than larger ones. The size of fragments can be determined by calibrating the gel, using the size standards, and comparing the migrated distance of the unknown fragment.