question archive In a mating of: Hfr pro+ tpr+ ile+ his + tet-s X F- pro- trp- ile- his- tet-r, cells are interrupted after several minutes and plated on the different media containing various amino acids shown below
In a mating of: Hfr pro+ tpr+ ile+ his + tet-s X F- pro- trp- ile- his- tet-r, cells are interrupted after several minutes and plated on the different media containing various amino acids shown below
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In a mating of: Hfr pro+ tpr+ ile+ his + tet-s X F- pro- trp- ile- his- tet-r,
cells are interrupted after several minutes and plated on the different media containing various amino acids shown below. The tet marker is known to be transferred very late and is used as a counterselected marker. The amino acids in the growth media and the number of colonies observed each as follows:
proline + tryptophan + isoleucine 440 colonies
histidine + tryptophan + isoleucine 290 colonies
proline + isoleucine + histidine 105 colonies
histidine + tryptophan + proline 165 colonies
What is the order of gene transfer (1st 2nd 3rd 4th)?
A.pro trp ile his
B.trp his pro ile
C.his ile pro trp
D.his pro ile trp
E.ile trp his pro
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Answer - D.his pro ile trp
Step-by-step explanation
The proline + tryptophan + isoleucine shows higher colonies 440. So without histidine
we got 440 colonies. So, histidine enter the first in F-.
In histidine + tryptophan + isoleucine shows second highest colonies 290.
So without Proline we got 290 colonies. So, proline enter the second in F-.
In histidine + tryptophan + proline shows third highest colonies 165.
So without Ile we got 165 colonies. So, Ile enter the third in F-.
In proline + isoleucine + histidine shows final lowest colonies 105.
So without Trp we got 105 colonies. So, trp enter the finally in F-.
