In a mating of: Hfr pro+  tpr+  ile+  his +  tet-s  X  F- pro- trp-  ile-  his-   tet-r, cells are interrupted after several minutes and plated on the different media containing various amino acids shown below

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In a mating of: Hfr pro⁺  tpr⁺  ile⁺  his ⁺  tet-s  X  F- pro- trp-  ile-  his-   tet-r,

cells are interrupted after several minutes and plated on the different media containing various amino acids shown below. The tet marker is known to be transferred very late and is used as a counterselected marker. The amino acids in the growth media and the number of colonies observed each as follows:

             proline + tryptophan + isoleucine           440 colonies    

                        histidine + tryptophan + isoleucine         290 colonies

                        proline + isoleucine + histidine              105 colonies

                        histidine + tryptophan + proline             165 colonies

What is the order of gene transfer (1^st 2^nd 3^rd   4^th)?

A.pro trp ile his

B.trp his  pro ile

C.his ile pro trp

D.his pro ile trp

E.ile trp his pro