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question archive You are performing an enzyme reaction that works the same way as your wheat germ acid phosphatase (WGAP) assay

You are performing an enzyme reaction that works the same way as your wheat germ acid phosphatase (WGAP) assay

Subject:ChemistryPrice: Bought3

You are performing an enzyme reaction that works the same way as your wheat germ acid phosphatase (WGAP) assay. First, you dissolve 50.0 umol of product into 1.00ml of buffer, and measure an absorbance of 0.800. Your cuvette has a 1.00cm pathlength. Next, you perform the enzyme assay. Your enzyme solution has a concentration of 50.0 ug/ml. 1) Mix 0.250ml substrate buffer with 0.250ml enzyme 2) Incubate 20.0 min 3) Stop the assay with 0.500ml NaOH 4) Read an absorbance of 0.400 * The units required for each answer in this problem are given. Answers to all three parts should be rounded to the appropriate number of significant digits* What is the extinction coefficient of the product? ml/umol/cm What is the catalytic activity measured in your assay? umol/min What is the specific activity measured in your assay? umol/min/ug Question code 4

 

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