question archive Problem 3) You are a researcher interested in examining the kinetics of starch digestion in the small intestines of patients with below average starch absorption
Problem 3) You are a researcher interested in examining the kinetics of starch digestion in the small intestines of patients with below average starch absorption
Subject:PhysicsPrice: Bought3
Problem 3) You are a researcher interested in examining the kinetics of starch digestion in the small intestines of patients with below average starch absorption. You know that the typical kinetic constants of amylase in the human body are kcat = 1 s-1 and km = 2.11 x 103 mg/mL. You take primary samples of intestinal enzymes from two patients, one with and one without issue and measure the initial rate of reaction in a 3x106 mg/mL starch solution. You find that in the healthy sample, the rate of reaction is 1.81 mg/mL-s whereas the patient with the absorption issue is 0.89 mg/mL-s. Using this information: a. Assuming Michaelis-Mentin kinetics, calculate the initial concentration of amylase in the samples from both the healthy and deficient patient (in mglmL). b. Name two (2) potential reasons for the difference in your answers in part a. c. Imagine a new scenario in which the initial enzyme concentrations were both 5 mg/mL, but the rates of reaction were the same. Using a typical Lineweaver-Burke plot, you find that Km = 1.54 x 10-3 mg/mL in the patient with below average starch absorption. Using this value and the original Km for the healthy patient, calculate kcat (in 5-1) for both. (:1. Name one (1) potential reason for the difference between your answers in part c.
