question archive Suppose you cut two different DNAs: one with Bam 3'-CCTAG G-5' and one with Bgl 3'-TCTAG A-5' H1: 5'-G GATCC-3' II: 5'-A GATCT-3' then ligate them together through their compatible sticky ends
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H1: 5'-G GATCC-3'
II: 5'-A GATCT-3'
then ligate them together through their compatible sticky ends. Once joined, could you separate these two DNAs again with either restriction enzyme? Why or why not? (Each of these enzymes is cut between the first two nucleotides, at the 5′ end of each strand (as indicated) of the palindrome.