I need help to answer all this questions   1

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I need help to answer all this questions
 

1. What characteristics must an organism have to be classified as GRAS?

2. What are the advantages and disadvantages of natural and transgenic proteins?

3. Explain the different expression systems. What are the advantages and disadvantages?

4. Choose an expression system for each case and explain why you think it is the right one.

a. They want to express a recombinant human antibody against COVID-19

b. It is desired to express a glutenase (enzyme that degrades gluten) which is a homoprotein that does not have disulfide bonds.

5. When we are going to extract protein from a cell we must be careful, because proteins are fragile, very similar. What does this mean?

6. Name and explain the different methods to extract protein. What are your advantages and disadvantages?

7. Explain the different methods to quantify proteins. What are your advantages and disadvantages?

8. Name and explain the different methods to concentrate protein. What are your advantages and disadvantages?

9. Design a method for protein extraction for the following examples.

a. If you want to recover a transgenic protein expressed in E. coli that accumulates in an inclusion body.

b. It is desired to recover a soluble protein in the cytoplasm expressed in the tobacco plant.

c. You want to recover a transgenic protein secreted by a yeast.

10. Explain how posttranslational modifications influence the selection of expression systems.