question archive Write 8 pages thesis on the topic the advantages and disadvantages of capillary electrophoresis
Write 8 pages thesis on the topic the advantages and disadvantages of capillary electrophoresis
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Write 8 pages thesis on the topic the advantages and disadvantages of capillary electrophoresis.
The main applications of the said technology include the field of analytical chemistry, biochemistry, biotechnology, forensic science, food science, clinical science, neuroscience, medical research and production, pharmaceutical science and other fields of disciplines (MicroSolv Technology, 2005). Due to the importance of the CE technique in different fields of application, the study is undertaken to present a comparative analysis of the said technique with other high-resolution separation techniques especially for application in different molecules of biological significance.
The main focus of the study includes prior research related to molecules such as proteins, DNA, steroids or non-polar molecules and chiral molecules. The development of the technique of separation referred to as capillary electrophoresis (CE) occurred years ago but the advent of its use in a multitude of fields occurred in the last decade. During the early years of introduction of CE, the main role of the said technique is related to educational and exploratory objectives. Through the course of its history, the importance of the said method in different analytical purposes paved the way to the present role in different fields for analysis of complex molecules (Camilleri, 1998).
This is important since the main focus of the research undertaken involves complex biologically significant molecules. The role of CE in the present era had been established on the basis of the capabilities of the said method. Included in such capabilities is adaptability in different modes of operation due to the simplicity of the method and high level of recovery and reproducibility. This can be attributed to the sharp separation of the components of analytes due to lower zone broadening. The advantages of the CE can be considered on the basis of the capability to allow high voltages for a higher rate of heat dissipation (Camilleri, 1998).
Due to the promising and significant results associated with the use of capillary electrophoresis, the study is targeted to present the positive and negative point in using the method for the analysis and separation of the different components of proteins, DNA, steroids or non-polar molecules and chiral molecules as compared to conventional procedures that are used to analyze these biologically significant molecules.
