question archive As described in the textbook, the plasmid vector pBluescript II contains an ampicillin resistance gene and a lacZ gene, within which is located a multiple cloning site
As described in the textbook, the plasmid vector pBluescript II contains an ampicillin resistance gene and a lacZ gene, within which is located a multiple cloning site
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As described in the textbook, the plasmid vector pBluescript II contains an ampicillin resistance gene and a lacZ gene, within which is located a multiple cloning site. How does one select for/identify bacterial cells containing recombinant (i.e., plasmids with inserts, not just plasmids) pBluescript II molecules?
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The multiple cloning site sequence is located in a lacZ-controlled gene engineered to provide a blue coloration when expressed in bacteria. This is typically accomplished by X gal contained in agarose growth media used to develop PBS bacteria. If the gene is disrupted by the active insertion of a DNA sequence (gene of interest), the bacteria display a white coloration in the Blue white screening. Distinguishing successful recombination from those phage centers that have not been changed.
