question archive 3) Purple eye color is hypothesized to be X-linked recessive in Drosophila melanogaster (fruit flies)

3) Purple eye color is hypothesized to be X-linked recessive in Drosophila melanogaster (fruit flies)

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3) Purple eye color is hypothesized to be X-linked recessive in Drosophila melanogaster (fruit flies). The P1 females were homozygous wild type (red eyes) but the males had purple eyes. There were 49 wild type males and 70 wild type females in the F1 generation. The F2 generation contained 32 wild type males, 73 wild type females, 40 purple-eyed males and one purple-eyed female. a. What is your null hypothesis? b. Does this data support or reject the hypothesis? Use chi square to prove your position.

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  1. Null hypothesis: The expected phenotypic ratio of F3 offspring with red eyes to purple eyes is 3:1
  2. based on our x2 value from chi-square test, we accept the null hypothesis at P=0.05. Our observed data produced a x2 value of 0.73972, which is less than the P value at 1df of 3.85. This indicates that the difference between expected and observed number of offspring with red/purple eye is statistically insignificant, and that there is a <5% probability that the observed ratio occurred due to chance.

 

  • note that degree of freedom (df) is calculated by subtracting 1 from our number of phenotypes. So on this case it's 2-1=1

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