question archive How do you compare the purity of the DNA samples ran on agarose gel electrophoresis based on their absorbance at 230nm and 280nm? It is said that at 230nm, its ratio from the absorbance at 260nm should be greater than 1
How do you compare the purity of the DNA samples ran on agarose gel electrophoresis based on their absorbance at 230nm and 280nm? It is said that at 230nm, its ratio from the absorbance at 260nm should be greater than 1
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How do you compare the purity of the DNA samples ran on agarose gel electrophoresis based on their absorbance at 230nm and 280nm? It is said that at 230nm, its ratio from the absorbance at 260nm should be greater than 1.5, while the ratio of 260nm to 280nm should be greater than 1.85nm. What if both samples are greater than the said values? Which sample would be considered as the purer one, the sample with higher ratios or the samples nearer to the said values?
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