All of the molecules listed are required to amplify a "target DNA segment" in vitro rapidly EXCEPT _______________

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All of the molecules listed are required to amplify a "target DNA segment" in vitro rapidly EXCEPT _______________.

Group of answer choices

 

primer

 

deoxyribonucleotides

 

restriction enzyme (endonuclease)

 

heat-stable DNA (Taq) polymerase

 

How does the CRISPR-Cas9 technique improve on the traditional genetic engineering method?

Group of answer choices

 

It uses specialized enzymes to cleave the DNA in vitro, releasing transposons ("jumping genes").

 

The cloning vectors are much more easily able to transfer genes of interest into target cells.

 

The premise is wrong; it does not really improve traditional methods. It is simply an alternative method.

 

Short guide RNAs associated with Cas9 are easily "programmed" to cut specific targets in the DNA to be edited.

Problems associated with the nuclear transplantation technique for cloning such as low efficiency and high incidence of abnormalities are most likely due to ________________ in adults.

Group of answer choices

 

cell-cell incompatibility

 

accumulation of epigenetic changes & shortened telomeres of the DNA

 

harmful mutations

 

ineffective use of enucleated egg

A __________ is a self-replicating DNA molecule used to carry genes from one organism to another.

Group of answer choices

 

DNA probe

 

recombinant DNA

 

cloning vector

 

restriction enzyme

Why are the wells containing DNA samples placed at the negative end of the gel electrophoresis apparatus?

Group of answer choices

 

 

Since DNA is a small molecule, samples will easily move through the gel.

 

DNA always diffuses towards the other side of the gel.

 

Since DNA is negatively charged, samples will move away from the negative side & travel to the positive side of the gel.

 

Since DNA is positively charged, samples will travel towards the negative end of the gel.