question archive In a chromatographic experiment you use a C18 column with a mobile phase mixture of acetonitrile and water (30:70 mixture)
In a chromatographic experiment you use a C18 column with a mobile phase mixture of acetonitrile and water (30:70 mixture)
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In a chromatographic experiment you use a C18 column with a mobile phase mixture of acetonitrile and water (30:70 mixture). After preliminary testing you determined that your analytes are well separated but the retention time is too long and peaks are too broad. What is your next step optimising chromatographic conditions?
A. Increase the column temperature by 100°C.
B. Change the injection volume.
C. Change the mobile phase mixture to increase the fraction of acetonitrile.
D. Change the mobile phase mixture to increase the fraction of water.
E. Change the stationary phase of the column to make it less polar.
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