question archive QUESTION 9 Suppose that there is a mutation (change in the DNA sequence) for this gone

QUESTION 9 Suppose that there is a mutation (change in the DNA sequence) for this gone

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QUESTION 9 Suppose that there is a mutation (change in the DNA sequence) for this gone. The original sequence was 3-GGCCATTTTTACGATAAA ATACCG-5 5' CCGGTAAAAATGC TATTTTATGGC-3' The new sequence is shown below; the underlined sections in the original and new (mutant) DNA highlight the point where there was a change (a C in the top strand and a in the bottom strand were deleted) 3* - GOCCATTITTAG ATAAAAACCG-5 5- CCG GTAMAA ATC TATTTTATGGC-3 How would this mutation affect the amino acid sequence in the polypeptide? The mutation introduces a stop codon near the start of the polypeptide. Instead of translating all of the mRNA and producing the normal polypeptide, translation will only produce the first small portion of the mRNA The original start codon (AUG) will be missing from the mRNA, so the first few amino acids will not be present in the polypeptide. Translation will start at the next AUG in the mRNA. In this case, that wil shift the reading frame and result in a completely different polypeptide being synthesized. The original start codon (AUG) is unaffected by this mutation, but the mutation deletes one nucleotide and therefore shifts the reading frame of the codons. The first few amino acids will be the same, but the amino acids following the deletion will be incorrect compared to the original There is one codon that is altered, but the rest of the mRNA is still intact. One amino acid will be changed, but the rest of the mRNA will be translated exactly as before This will have no effect whatsoever on the polypeptide. The change occurs at the third position of a codon and calls for the same amino acid as the original sequence. The amino acid sequence of the polypeptide is unchanged. This wil have no effect whatsoever on the polypeptide. It is only a one nucleotide change, and that is much too small of a change to substantially affect the structure of the polypeptide

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