question archive Let's assume that you want to generate a recombinant plasmid by inserting the bacterial Gene 123 into the shown plasmid
Let's assume that you want to generate a recombinant plasmid by inserting the bacterial Gene 123 into the shown plasmid
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Let's assume that you want to generate a recombinant plasmid by inserting the bacterial Gene 123 into the shown plasmid. a. Which restriction enzyme(s) would you use to digest both the insert and plasmid? b. After the digestion is completed and ligation joins the fragments together, E. coli cells are transformed with the ligation product. Assuming that you can have a mixture of ligation products, e.g. insert to insert ligation, insert to plasmid ligation, etc., it's necessary for you to isolate bacteria that have been transformed with the plasmid. What growth condition can you use to separate cells carrying the whole plasmid from those carrying only an insert? C. How can you select bacteria carrying a plasmid with an insert versus those carrying a plasmid without the insert? Explain how would you achieve it. EcoR I Kpn I Kpn - BornHI | Xho I BamHI Gene 123 Nde I Click Save and Submit to save and submit.
