question archive 1) explain why it is necessary to include only one chain-terminating/synthesis-terminating nucleotide in each well of the electrophoresis instrument? 2) Greider and Blackburn (1987) purified telomerase activity by chromatography, and they identified some DNA oligonucleotide primers that would get a DNA-addition reaction started
1) explain why it is necessary to include only one chain-terminating/synthesis-terminating nucleotide in each well of the electrophoresis instrument? 2) Greider and Blackburn (1987) purified telomerase activity by chromatography, and they identified some DNA oligonucleotide primers that would get a DNA-addition reaction started
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1) explain why it is necessary to include only one chain-terminating/synthesis-terminating
nucleotide in each well of the electrophoresis instrument?
2) Greider and Blackburn (1987) purified telomerase activity by chromatography,
and they identified some DNA oligonucleotide primers that would get a DNA-addition reaction started.
a) In the addition reaction, they labeled certain nucleotides, and ran the products on a gel. They showed that certain oligonucleotides primers (like (TTGGGG)3) had one pattern but other ones (like (GGGGTT)3) shifted the products up by multiple bases. How did they interpret this?
b) Based on the description in a, how do you expect products from a (TGGGGT)3) primer would compare to the first primer?
