How will you prepare Moleculary Imprinted surface with an af?nity to catalase enzyme using polyacrylamide as a polymer? Please write a step wise process. 2. Why is gold an excellent choice for preparation of smart nanomaterials? Elaborate your points. 3. What will you do to prepare graphene on industrial scale if you have a lot of graphite? State the process step wise along with rationale for each of your steps. 4. What is the significance of Quantum size effect in the development of biosensors? 5. How is the fluid flow controlled on the following? a. Paper based LoC b. Lab on a disc c. EMF-based Platforms 6. Why is digital PCR more effective for hunting mutated DNA instead of conventional PCR? 7. We know that in cantilever based biosensors for cell counting the change in mass of the cantilever is deduced from the change in frequency of vibration. But how do we measure the frequency of vibration? Also explain different variables that effect the consistency of data produced by cantilevers. 8. Differentiate between LoD and Lot). and explain their significance in Biosensors. 9. Why are synthetic antibodies superior to natural antibodies? How do we synthesize antibodies? 10. Draw, label and elaborate MEMS coulter counter.

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