question archive Hint) this is a fairly challenging question
Hint) this is a fairly challenging question
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Hint) this is a fairly challenging question. Remember that a single bp change mutation can change the target site of a regulator. It can also alter a protein to change its properties.
In a well characterized Oc mutant strain, mutagenesis induces a second mutation that restores the wild-type phenotype. You determine that this second mutation does not affect the O site (doesn't change operator DNA sequence). Furthermore, you find that it acts in trans and it is dominant to its wild-type, allele. Your next candidate is:
a) lacI
b) CAP
c) all the others are candidates
d) lacP
e) lacZ
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a) lacI
Step-by-step explanation
Inducer remains the regulatory site of maintaining expression of lac operon. The inducer can act as a cis or trans acting element on the cistron by modulating secondary binding of the repressor on the operator. Thus any faulty operator can be rectified using a suitable inducer. Over- expressing operator can be down-regulated using a suitable inducer.
